Sentence examples for blot analysis were prepared from inspiring English sources

Yeast samples for western blot analysis were prepared according to Zabrocki and co-authors [41].

Whole cell extracts for Western blot analysis were prepared by TCA precipitation.

Whole-cell extracts for Western blot analysis were prepared from freshly sorted c-kit+ cells after a 5-h incubation in StemSpan medium with growth factors alone or together with CB. 30 µg extracts were prepared from washed cells in 100 µl extraction buffer.

Cell lysates for western blot analysis were prepared 72 h after siRNA transfection.

Lysates for western blot analysis were prepared from cells and aortic tissues.

Whole-cell lysates or nuclear extracts used for Western blot analysis were prepared as previously described in detail [ 40, 41].

Eg5 bands (as judged from parallel western blot analysis) were excised and prepared for tryptic digestion and MS. Gel slices (1 2 mm) were prepared for mass spectrometric analysis using the Janus liquid handling system (Perkin-Elmer, UK).

For western blot analysis, extracts were prepared in the presence of protease inhibitors as previously described [16], and results are representative of experiments repeated at a minimum of three times.

For western blot analysis, lysates were prepared in disruption buffer (6 M urea, 2 M β-mercaptoethanol, 4% SDS), sonicated to shear nucleic acids, and boiled for 5 min prior to polypeptide separation by SDS-PAGE on 4 15% Tris-HCl gradient gels Bio-Rad Laboratoriess, CA, USA).

Western blot analysis Lysates were prepared from myotubes transfected with siRNA against a scrambled sequence or SNARK.

For Western blot analysis, samples were prepared in Laemmli's buffer, heated for 5 min at 95 °C, and equal amounts of protein (30 μg) were loaded into Novex® 10 20 % Tricine gels.