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Protein lysates and western blot analysis were preformed as previously described [64].
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Densitometry analysis were preformed and normalized with α-tubulin and then presented as percentage of control.
Aliquots of equal volume were examined using western blot analysis for the presence of sema3s using antibodies directed against the appropriate tags as previously described [19] and the densitometry analysis was preformed using MultiGauge software (FUJIFILM).
Statistical analysis was preformed with SAS 9.2 (Cary, NC) for Windows.
To confirm that ASL3 was the gene associated with the phenotype observed, RNA interference (RNAi) analysis was preformed.
The inelastic time history analysis is preformed using the direct integration technique considering a time step of 0.005 s.
Statistical analysis was preformed using SPSS 13.0 for MicroSoft™ Windows.
All slides were kept at 4°C in dark until confocal microscopy analysis was preformed.
Statistical analysis was preformed using student's t-test (SPSS. Chicago, IL).
Univariate analysis was preformed, separately, on each morphometric parameter, using ANOVA (analysis of variance), with STATISTICA software.
Loci-spanning probe melt analysis was preformed by continuous signal acquisition between 38°C – 65°C at default ramp rate of 0.6°C/s and 5 acquisitions per °C.
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