Sentence examples for blot analysis were obtained from inspiring English sources
The reagents used in the Western blot analysis were obtained from Sigma.
The reagents used in the electrophoretic mobility shift assay (EMSA) and Western blot analysis were obtained from Sigma.
The results for western blot analysis were obtained using the Student t test.
All primary antibodies used for Western blot analysis were obtained from Cell Signaling Technology (Beverly, MA) unless otherwise stated and concentrations used were according to the manufacturers' recommendations.
Whole cell extracts for western blot analysis were obtained from alginate beads, from medium (containing the emigrated, swimming spheroids) and from adhered colonies.
The probe for Northern blot analysis was obtained by EcoRI digestion of pCRII TOPO-COQ5 and was labeled and purified as described in [19] and hybridized to a commercial membrane (FirstChoice Human Blot 1 membrane Ambion) containing 2 μg/lane of poly(A) + RNA from ten human tissues, previously used as described [23] and stripped.
The data of cardiac performance, subcutaneous microcirculation, and Western blotting analysis were obtained from at least six independent experiments.
The data of cell viability assay were obtained from at least six independent experiments, each done in triplicate, whereas the data from Western blotting analysis were obtained from at least six independent experiments.
Furthermore, with the exception of those from complete genome analyses (Additional File 2, Additional Table S1), the individual genomic sequences included in the analysis were obtained via Western blots using an antibody from a similar LHC [e.g. [ 50]], Southern blots using a probe from a similar LHC [e.g. [ 51]] or PCR using primers based on an LHC alignment [e.g. [ 52]].
Results obtained from Western blot analysis were statistically evaluated with one-way ANOVA and Tukey's comparison multiple range tests with GraphPad Prism™ version 4.0 software (GraphPad Software Inc., San Diego, USA).
Results obtained by Western Blot analysis were further confirmed by an optimized in-house enzyme-linked immunosorbent assay (ELISA).
