Sentence examples for blot analysis were extracted from inspiring English sources

Protein samples for western blot analysis were extracted and separated as described previously.

MK-571 was purchased from Merck Sharp (Kirkland, Canada) Proteins for Western blot analysis were extracted by lysing cells with sample buffer containing 0.125  M Tris-HCl, 2% SDS, 10% glycerol and 0.001% bromophenol blue.

RNA for northern blot analysis was extracted with Trizol (Sigma) using the manufacturer's recommendations.

Total RNA for Northern Blot analysis was extracted with a LiCl method [ 52] from T. borchii mycelia grown for: a) 30 min, 24 h and 29 h or under of NaCl treatment; b) 48 h of cold treatment; c) 5 h on CM after 24 h of NaCl treatment and d) 30 days at 24°C on CM.

Concerning Western blot analysis, proteins were extracted from jejunal mucosa scrapings as described above.

For western blot analysis, proteins were extracted as described above.

For Western blot analysis, proteins were extracted from normal and tumour tissues in five representative breast cancer cases.

For western blot analysis, proteins were extracted by scraping the NPCs in homogenate buffer (320 mM sucrose, 1 mM EDTA, 50 mM Tris-HCl, 1 mM PMSF and 1 × proteinase inhibitor).

For Western blot analysis cells were extracted with RIPA buffer (1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 5 μg/ml aprotinin, 5 μg/ml leupeptin) for 20 min on ice and centrifuged at 14 000 rpm at 4°C. SDS-polyacrylamide (12%) gel electrophoresis of 15 μg supernatant protein was performed.

Whole cell extracts for Western blot analysis were prepared by TCA precipitation.

Isolation of nuclear extracts and Western blot analysis were performed as described previously [ 14].