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Quantitative real-time polymerase chain reaction arrays and Western blot analysis were employed to identify the cell-cycle control- and apoptosis-related genes regulated by CENP-A.
SDS-PAGE and Western blot analysis were employed to determine the enrichment of serum.
RT Q-PCR and Western blot analysis were employed to investigate the activities of AMPK, FOXM1 and AKT/FOXO3a signaling.
Spectrofluorimetry, circular dichroism (CD) studies, in vitro histone acetyl transferase (HAT) assay and western blot analysis were employed as experimental tools.
Real-Time PCR and Western blot analysis were employed to validate the changes in expression of relevant genes including the protein levels of C3 which is a component of the complement cascade.
In addition, both IHC and Western blot analysis were employed to evaluate levels of derlin-1 expression in another set of tumor samples with paired normal breast tissues from 13 patients.
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Western blot analysis was employed to evaluate levels of AMPK, mTOR, raptor, Beclin-1, p62 and LC3-II.
Western blot analysis was employed to quantify total numbers of hippocampal NR2A and NR2B.
The same anti VILIP-1 antibody used in Western blot analysis was employed as primary antibody at 1/500 and 1/1000 dilutions.
Western blot analysis was employed to quantify GFP expression.
Western blot analysis was employed to determine the protein levels of two autophagy markers, Light Chain 3 (LC3) and beclin‐1.
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blot analysis were utilised
blot analyses were employed
blot analysis were used
blot analysis were confirmed
blot analysis were euthanized
blot analysis were prepared
blot analysis were purchased
blot analysis were detected
blot analysis was employed
blot analysis were preformed
blot analysis were obtained
blot analysis were harvested
blot analysis were separated
blot analysis were carried
blot analysis were described
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