Exact(1)
For determination of splenic reverse transcriptase activity, 10% homogenates of spleen samples prepared for Western blot analysis were diluted 1∶1000 and subjected to PERT assays as described elsewhere [36] (making use of a modification for real-time detection).
Similar(59)
The antibodies used for Western blot analysis were all diluted in Tris buffered saline (TBS) buffer (20 m M Tris-HCl, 137 m M NaCl2 and 0.05% Tween 20) supplemented with 5% skim milk powder (Merck, Darmstadt, Germany).
The western blot analysis was performed with mouse serum diluted 1 100 and probed with an alkaline phosphatase-conjugated goat anti-mouse IgG Fc (Jackson ImmunoResearch, Avondale, PA, USA).
Lectin blot analysis was performed as previously reported (Yamamoto et al, 2009), using HRP-labelled Datura stramonium agglutinin (DSA; Seikagaku, Tokyo, Japan) diluted 1 : 2000, and DSA recognises β1-4GlcNAc branching.
Western blot analysis was performed to study the magnetic fraction.
Western blot analysis was used to determine the SG modulators.
Western blot analysis was performed as described above.
Western blot analysis was used to confirm protein expression.
Western blot analysis was performed.
Western blot analysis was repeated three times.
Northern blot analysis was performed as described[7].
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