Immunoprecipitation and direct Western blot analysis were used to detect interactions between these proteins and their total expression, respectively, whereas interactions on chromosomal arms were detected using a trapped in agarose DNA immunostaining assay.
Immunoprecipitation and western blot analysis were used to detect MyD88s protein expression as indicated by the arrow (27 kDa) (g).
To examine expression of Capn4 in primary NPC tumor and normal nasopharyngeal tissues, RT-PCR and Western blot analysis were performed to detect mRNA and protein levels.
Real-time PCR and Western blot analysis were performed to detect Notch-1, Notch-2, Notch-3 and Notch-4 receptor expression in breast cancer cells when PEA3 was knocked down by siRNA.
To further investigate Plexin-B1 expression in ovarian cancer cells, RT-PCR and western blot analysis were used to detect Plexin-B1 mRNA and protein levels in four human ovarian cancer cell lines: SKOV3, A2780, C13* and OV2008.
Protein complexes bound to the beads were washed seven times with lysis buffer containing 0.5% octyl-glucoside, then eluted in sample buffer (50 mM Tris, pH6.8, 2% SDS, 5% β-mercaptoethanol, 10% glycerol, 0.01% BPB) at 80°C, 5 min. SDS PAGE and western blot analysis were used to detect Vac14 HA or V5, Vac7, Fab1, Fig4 Myc.
A loss of H2BK37me2 signal by Western blot analysis was not detected upon deletion of the individual candidates, as was observed for the control H2B K37R and H2B K37A mutants compared to their isogenic strain expressing wild-type H2B (Figure 3A, bottom).
To determine which form of LC3 is affected by the presence of Cas III-ia, Western blot analysis was used to detected LC3-I and LC3-II levels.
Western blot analysis was performed and GAPDH was detected using antiGAPDH monoclonal antibody (Chemicon International).
Western blot analysis was performed to detect MMP-2 and MMP-9 protein expression.
