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When more than two-fold changes in expression level by Northern blot analysis were considered significant, consistency with cDNA microarray analysis were 55% (41 of 74), whereas no significant change was seen in 27% (20 of 74), and discordant results were obtained in 18% (13 of 74).
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Total RNA extraction and northern blot analysis were performed.
The intensities of LDHA bands from Western blot analysis were estimated by densitometric analysis.
Preparation of total cell lysates and Western blot analysis were performed as described previously.
Protein extraction and western blot analysis were carried according to our previous procedure33.
Traditionally, Southern blot analysis has been considered the most accurate method to size the full mutation and to determine the methylation status of the expanded alleles for all mutation sizes.
Western blot analysis was performed to study the magnetic fraction.
Southern blot analysis was performed five times.
To measure FtsZ protein levels, western blot analysis was performed.
Western blot analysis was used to confirm protein expression.
Western blot analysis was performed to assess ERK activity and efficacy of MEK inhibition.
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