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Cellular protein extraction and western blot analysis were carried out as described (Supplemental Information).
RT qPCR and Western blot analysis were carried out as previously described Kallen et al (2013).
Immunohistochemistry and Western blot analysis were carried out according to the previous report.
Protein extraction and Western blot analysis were carried out as described [17].
An enzyme assay, sodium dodecyl sulfate (SDS -polyacrylamide gel electrophoreSDS -polyacrylamidet analysis were carried out as described previously [ 19].
32 To confirm the E7, Dnmt1, Dnm3a, and Dnmt3b protein level in E7 and empty plasmid-transfected C33a cells, Western blot analysis were carried out as previously described.
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Northern blot analysis was carried out as described by Sambrook et al. (1989).
Western blot analysis was carried out following the procedure described in Sakamoto et al. (2008).
a, b Western blot analysis was carried out after 48 h without (a) and with (b) treatment of 65.76 μM lipopeptide.
To identify transgene-positive plants, the transgenic plants were examined by PCR with the primer pair (gusAF: 5′-ACGACTCGTCCGTCCTGTAG-3′ and gusAR: 5′-CCGCATCACGCAGTTCAA-3′). To determine the copy number of the transgene, Southern blot analysis was carried out.
Consequently, Western blot analysis was carried out with these species.
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blot analysis were quantified
blot analysis were confirmed
blot analysis were performed
blot analysis were used
blot analysis were euthanized
blot analysis were prepared
blot analysis were detected
blot analysis were purchased
blot analysis were homogenised
blot analysis were diluted
blot analysis was carried
blot analysis were employed
blot procedure were carried
blot analysis were preformed
blot analysis were harvested
blot analysis were obtained
blot hybridization were carried
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