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Quantitative real-time PCR (qPCR) and western blot analysis were applied to validate gene expression profiling.
SDS-PAGE and Western blot analysis were applied to analyze the purity and to identify the recombinant swollenin.
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Western blot analysis was applied to two identified markers to validate the expression of intact proteins by an antibody-based approach and observed by mass spectrometric analysis using expression of digested peptides.
Real-time PCR was used to quantify the expression of Axin, and western blot analysis was applied to measure protein levels of Axin, β-catenin, Cyclin D1, MMP-7, DNMTS, MeCP2 and acetylated histones.
To confirm proteins identified by RP-nano-UPLC-ESI-MS/MS, Western blot analysis was applied to detect the candidate protein that may be associated with cell adhesion/growth pathways on the CNTs/SF polymer surface.
In order to further test the hypothesis of differentiation defects and functional alterations due to the down-modulation of the CD11b integrin, Western blot analysis was applied to differentiation markers, kinase expression and metabolic factors.
Densitometry analysis was applied to each blot to quantify the intensity of bands, followed by normalization against loading controls.
Thematic analysis was applied.
Content analysis was applied.
Northern blot analysis was also applied for testing if 4 of 19 released but unknown sequences were present in human epididymis.
Dot blot and Western blot analysis were performed as described [46].
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