Western blot analysis was used to confirm protein expression.
Western blot analysis was used to visualize the phosphorylation of ERK1/2, a direct indicator of RAS pathway activation.
Western blot analysis was used to evaluate essential autophagy markers, Akt and AMPK, and the downstream signal mTOR.
Western blot analysis was used to evaluate intracellular Ca2 + regulatory and mitochondrial proteins, PKA and its downstream signal eNOS.
TF antigen was determined with immunohistochemistry. Northern blot analysis was used to determine steady- state TF messenger RNA (mRNA).
Western blot analysis was used to compare SnSAG expression in merozoites versus sporozoites, while immunocytochemistry was performed to examine expression of the SnSAGs in merozoites versus bradyzoites.
Western blot analysis was used to detect the protein expression of Heat shock protein 27, focal adhesion kinase, p38 MAPK and their phosphorylation levels in rat cardiac muscle after 14d hindlimb unloading.
Western blot analysis was used to determine the SG modulators.
Northern blot analysis was used to assess if PTP1B overexpression was coincident with increased transcription of the PTP1B gene.
Western blot analysis was used to quantify the levels of endothelial-derived nitric oxide synthase (eNOS) and cyclooxygenase II (COX II).
Dot blot analysis was used to determine qualitatively whether the VRC01 was expressed into the supernatant (Fig. 2).
