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Western blot analysis was repeated three times.
Northern blot analysis was repeated three times using antler tips from three individual deer.
These clones were further expanded and following digestion of DNA with NdeI, Southern blot analysis was repeated using a 790 bp probe (3' external probe), which detects a 10.9 kb fragment derived from the wild-type Mro allele and a 7.1 kb fragment from the correctly targeted allele.
The western blot analysis was repeated 3 times.
Western blot analysis was repeated at least three times.
Western blot analysis was repeated at least twice for each experiment.
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We wish to emphasize that the experiments on CYP2J3 overexpression with Western blot analysis were repeated 4 times, with identical results obtained each time.
Western analysis was repeated four times.
The Northern blot analysis was here repeated with a larger number of tissues and a probe containing a 3' portion of the gene (exon 28). Figure 2 shows results of the three different Northern blots used.
Quantitative, two-colour Western blot analysis was performed on three biological repeats using a LICOR Odyssey FC instrument and Image Studio V2.0 analysis software.
Southern blot analysis was performed five times.
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