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Nevertheless, as shown in Fig. 3, 1D Western blot analysis was not applicable in detecting age-related changes in protein sulfenation because the 1D approach yielded a very low resolution that could not produce well-defined and distinguished bands for protein identification.
A loss of H2BK37me2 signal by Western blot analysis was not detected upon deletion of the individual candidates, as was observed for the control H2B K37R and H2B K37A mutants compared to their isogenic strain expressing wild-type H2B (Figure 3A, bottom).
However, our Western blot analysis was not able to detect significant antibody response at 8 and/or 12 kDa.
Similarly, the amount of PPAR γ protein, detected by western blot analysis, was not different in the two groups.
A potential limitation of this study is that tissue mRNA expression or protein activation by western blot analysis was not measured.
The remaining tissue had to be fully processed for RT-PCR and Western blot analysis was not included in our protocol.
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Correspondingly, the amounts of HA0 incorporated into pseudoparticles, as measured by western blot analysis, were not affected by the increase in HA NA plasmid ratio, while the amounts of particle-associated NA protein indeed increased (Figure 4A).
The AUC for the immune reactivity towards α-synuclein in early vs late PD patients evaluated by both ELISA and Western blot analysis were not significantly greater than 0.5 and the ROC curves were close to diagonals, respectively (data not shown).
The total FAK and β-actin, a loading control for western blot analysis, were not changed by SMYGT treatment.
Because Western blot analysis is not practical for daily routinely use in the clinic, we tested the ability of multiple reaction monitoring (MRM) to detect vinculin abundance in urine.
In this study slight discrepancies between used methods may exist, caused by the fact that the probes used in FISH and Southern blot analysis are not overlapping and thus cover another genomic region, although all map to the 19q12 13.2 region.
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