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Western blot analysis was employed to quantify total numbers of hippocampal NR2A and NR2B.
Western blot analysis was employed to evaluate levels of AMPK, mTOR, raptor, Beclin-1, p62 and LC3-II.
The same anti VILIP-1 antibody used in Western blot analysis was employed as primary antibody at 1/500 and 1/1000 dilutions.
Western blot analysis was employed to quantify GFP expression.
Western blot analysis was employed to determine the protein levels of two autophagy markers, Light Chain 3 (LC3) and beclin‐1.
A pull-down assay with glutathione S-transferase (GST -agarose beads followed by Western blot analysis was employed to confirm microtubule S-GST -agaroseation. Immunofluorescence microscopy using a mouse monoclonal anti-α-tubeads-followed used to study the effect of the S-glutathionylation on microtubyle function; mainly polymerization and depolymerization.
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Quantitative real-time polymerase chain reaction arrays and Western blot analysis were employed to identify the cell-cycle control- and apoptosis-related genes regulated by CENP-A.
SDS-PAGE and Western blot analysis were employed to determine the enrichment of serum.
RT Q-PCR and Western blot analysis were employed to investigate the activities of AMPK, FOXM1 and AKT/FOXO3a signaling.
Spectrofluorimetry, circular dichroism (CD) studies, in vitro histone acetyl transferase (HAT) assay and western blot analysis were employed as experimental tools.
Real-Time PCR and Western blot analysis were employed to validate the changes in expression of relevant genes including the protein levels of C3 which is a component of the complement cascade.
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