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Western Blot analysis was conducted using the following primary antibodies and dilutions: anti-PTEN: 1 1000, ab154812 (Abcam, Cambridge, UK), anti-beta actin 1 2000, A0760 40 (USBiological, Massachusetts, USA) and anti-GAPDH 1 3000, ab9485 (Abcam), anti-CREB 1 250, 48H2 (Cell Signalling, Boston, Massachussets).
And Western blot analysis was conducted to investigate the expression levels of Bcl-2 and procaspase-3/-8/-9 proteins (Figure 6).
Western blot analysis was conducted as previously described [32].
Western blot analysis was conducted using a standard protocol.
Western blot analysis was conducted to ensure equal kinase protein expression.
In order to characterize the IKAP protein levels, a western blot analysis was conducted (Figure 1D).
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qRT-PCR and western blot analysis were conducted to confirm the overexpression of CBX6-FLAG in the stable cell line.
Western-blot analysis was conducted as already described [47].
The Northern blotting analysis was conducted as described previously [ 44].
Western blotting analysis was conducted by SDS PAGE on 4 15% polyacrilamide precast gels (Bio-Rad, Hercules, CA, USA).
Cell lysates were prepared in radioimmunoprecipitation assay (RIPA) lysis buffer (50 mmol/l Tris-HCl, 150 mmol/l NaCl, 1% NP-40, 0.5% SDS, and 1% deoxycholic acid) and the western blotting analysis was conducted as per a standardized procedure.
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blot hybridization was conducted
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blot analysis were conducted
blot test was conducted
blot analysis was repeated
blot analysis was also
blot analysis was applied
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