Sentence examples for blot analysis was applied from inspiring English sources
Phosphorylation in the PI3K-AKT-mTOR signaling pathway: Western blot analysis was applied to examine PI3K-AKT-mTOR pathway components potentially involved in P2X7R-enhanced PDLSC osteogenesis.
Upstream signals involved in the potential pathway: Western blot analysis (see Section Western blot analysis) was applied to evaluate protein expression profiles in PDLSCs (with Ad-P2X7R transfection or Ad-control transfection) in response to inflammation and/or addition of P2X7R agonist/antagonist.
To obtain quantitative data of these specific sites, the ligation-mediated polymerase chain reaction technique, instead of Southern blot analysis, was applied.
Western blot analysis was applied to observe the expression of osteogenic-related proteins after treatment with different concentrations of earthworm extract for 7 days.
Real-time PCR was used to quantify the expression of Axin, and western blot analysis was applied to measure protein levels of Axin, β-catenin, Cyclin D1, MMP-7, DNMTS, MeCP2 and acetylated histones.
Western blot analysis was applied to two identified markers to validate the expression of intact proteins by an antibody-based approach and observed by mass spectrometric analysis using expression of digested peptides.
The subtraction suppression hybridization (SSH) technique and northern blotting analysis was applied to explore the pluripotency-associated genes under a unique epigenetic sub-microenvironment.
Densitometry analysis was applied to each blot to quantify the intensity of bands, followed by normalization against loading controls.
