Sentence examples for blot analysis utilising the from inspiring English sources

AR status was confirmed in these cell lines by Western blot analysis utilising the same antibody (AR441) as that employed in the IHC method through the presence of a band at 110 kDa in LNCaP and the absence of a band in PC3 (Inset to Figure  1), in keeping with previously published studies [ 19].

Item analysis of questionnaires will be via Rasch analysis, utilising the WINSTEPS TM statistical software.

Variables were analysed using binary logistic regression analysis utilising the backwards stepwise elimination method.

Western blot analysis utilising antibodies for Bad and PARP also showed the induction of apoptosis by everolimus (Supplementary Figure 1); everolimus (20 n) increased the expression of Bad and cleaved PARP protein.

Therefore, we performed western blot analysis utilising anti-LC3A/B antibodies that preferentially bind LC3-II protein.

Analysis utilised the actuarial survival method (ie, age standardised relative survival).

Western blot analysis was utilised to determine the expression status of the MMTV-c- myc transgene in the mammary tumours and mammary gland tissue from parous study mice (c- myc transgene-negative mice were included as an assay negative control).

Immunoprecipitation and Western blot analysis were utilised to examine the potential association between erbB3 and IRS-1 in MCF-7, T47D and BT-474 cells in the absence and presence of the erbB3/4 ligand heregulin β1 (HRGβ1).

Similarly, BamHI-digested DNA from the transfected ES cells used for Southern blot analysis that utilised a 5′ probe, which consisted of a 1.4 kb XbaI– EcoRI fragment, yielded a wild-type allele of 17.4 kb and a mutant allele of 9.7 kb (Fig. 1B).

The HPLC analysis utilised a sub-sample of the extracts prepared for sugar analysis.

The analysis utilised survey responses of 53 Member States comprising the WHO European Region.