Sentence examples for blot analysis using total from inspiring English sources

Protein expression was confirmed by western blot analysis using total cell lysate or the P3 fraction.

To investigate whether VILIP-1 protein down-regulation was caused by silencing of transcription, we performed Northern Blot analysis using total RNA extracted from cells.

To examine the size of the kakusei transcript, we performed Northern blot analysis using total RNA isolated from whole brains of bees anesthetized with CO2 and bees awakened from CO2-induced anesthesia.

We also performed RNA blot analysis using total RNAs isolated from hygromycin-resistant and control calli and found that the OsSRT1 transcript level was significantly reduced in hygromycin-resistant calli (Figure 3A).

The size and expression levels of candidate sRNAs were assessed by Northern blot analysis using total RNA isolated from Bt CDC272.

Western blot analysis using total protein from normal pituitaries and pituitary tumours from Men1+/− mice was performed (Fig. 1G), as previously described (Nesbit et al. 2004).

We performed Northern-blot analysis using total RNAs isolated from the epididymides of sham-operated (wild-type control) mice, mice that had been bilaterally castrated and reared for 1 week, and mice that had been bilaterally castrated, reared for 1 week then treated with dihydrotestosterone (DHT).

To determine whether these proteins are present in MPM cells, we conducted a conventional western blotting analysis using total cell lysates of MSTO-Mock, MSTO-CD26WT or MSTO-CD26/10Chi MSTO-CD26/10Chi

The Western blot signals were quantitated by densitometric analysis using Total Lab Nonlinear Dynamic Image analysis software (MathWorks, Natick, MA, USA).

Intactness of the transgenes was carefully mapped by Southern blot analysis using a total of 11 different restriction enzymes and hybridization with cosmid LCRε, -γγδβ probes [16], and smaller probes along the β-globin locus [11].

After adenoviral infection, status of caspase-3 was monitored starting from 6 to 36 hours by Western blot analysis using antibodies against total caspase-3 (Figure 3C, top lane) or specifically cleaved caspase-3 (Figure 3C - middle lane).