Sentence examples for blot analysis using the from inspiring English sources

Then, isolated protein samples were subjected to 10% SDS-PAGE separation and subsequent Western blot analysis using the indicated antibodies.

The molecular weight of coPEDF was confirmed by western blot analysis using the conditions of Laemli.

Levels of different proteins were analyzed by Western blot analysis using the respective antibodies.

The mRNAs were detected by routine western blot analysis using the anti-T7 antibody.

The degree of Proteinase K digestion was visualized by Western blot analysis using the anti-FLAG antibody (Sigma).

Aliquots of the collected fractions (0.5 ml each) were subjected to Western blot analysis using the anti-FLAG M2 antibody.

This was confirmed by Southern blot analysis using the two wMel methyltransferase ORFs as probes (Fig. 1).

The presence of F. novicida Hfq protein was verified by Western blot analysis using the method described below.

Northern blot analysis using the antisense riboprobe revealed the expression of CYP3A43 mRNA in human brain (Fig 1b).

Next, we examined the activation of NFATc4 by western blot analysis using the lysates from IP3R1−/−-IP3R2−/ IP3R1−/−-IP3R2−/

Extracts were loaded on SDS-PAGE and the presence of endogenous TRAF4 was tested by Western blot analysis using the 2H1 mouse monoclonal antibody as previously described [7].