Sentence examples for blot analysis using standard from inspiring English sources

Precipitated proteins were washed and subjected to SDS-PAGE and Western blot analysis using standard procedures.

Protein quantification of type II collagen was conducted via western blot analysis using standard protocols.

Expression of AFP was analyzed by western blot analysis using standard procedures.

Equal amounts of total protein for each sample were subjected to Western blot analysis using standard protocols.

Quantification of NEDD8 and ubiquitin was performed by Western blot analysis using standard curves based on known amounts of purified protein.

Protein samples were separated by SDS NuPAGE 4 12% Bis-Tris PAGE (Invitrogen) transferred to nitrocellulose membrane and detected by Western blot analysis using standard techniques.

The following antibodies were used for immunoblotting analysis using standard Western blotting procedures: superoxide dismutase 1 (superoxideeroxidismutasease 2 (SOD2), p22phox, and p-p40phox were purchased from Santa Cruz Biotechnology, Dallas, TX, USA; β-actin, tublin, diphenyleneiodonium chloride (DPI), and capsaicin were purchased from Sigma-Aldrich, St . Louis MS, USA.

The suspension was clarified by centrifugation at 13,000×g for 30 min at 4 °C and subjected to western blot analysis using a standard procedure.

The specific C-ADP uptake rates (per mg AAC) were calculated following the quantification of wild type and mutant AAC by Western blot analysis, using a standard curve of purified MtAAC.

The ES cells with the correct recombination were confirmed by standard Southern blot analysis using the probe described above.

Another 2.5 OD600 units from the same culture were used to prepare protein for standard western blot analysis using an anti-human c-myc tag antibody (Biolegend) (Supplementary Material, Fig. S1).