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The yield of RNA silencing was assessed by western blot analysis using specific antibody.
RNA was extracted and measured by Northern blot analysis using specific rat GIP probes.
Preparation of total RNA and Northern blot analysis using specific RNA probes were performed as described previously [29].
Low levels of SOD expression was detected in B. neotomae strain 5K33 by Western blot analysis using specific polyclonal antibodies.
All colonies were pooled and examined for the expression of endogenous MBP-1 by Western blot analysis using specific antibody.
To investigate whether pathway linking p53 and senescence after knocking down MBP-1 in HFFs, we examined the post-translational modification of p53 by Western blot analysis using specific antibodies.
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AgPRS2,4 and AgPRS3 disruption was confirmed by Southern-blot analysis using specific radiolabeled probes (Fig. 3).
The quality of the samples was verified by Northern-blot analysis using specific digoxigenin-labelled riboprobes for each virus and evaluating both A260/230 and A260/280 absorption ratios (Nanodrop- Thermo Scientific).
The presence of MMP-1, MMP-2, MMP-9 in the media of normal fibroblasts, desmoid fibroblasts and desmoid fibroblasts plus toremifene was evaluated by Western-blot analysis using specific monoclonal antibodies (Fig. 5).
The expression levels of target proteins were measured by Western blotting analysis using specific antibodies, and α-tubulin mRNA levels were assessed by reverse transcription polymerase chain reaction (RT-PCR).
Transcription directed by each construct was confirmed by RT-PCR analysis and resulting cDNA products subjected to DNA gel blot hybridization analysis using specific probes.
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