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Western blot analysis using polyclonal anti-AH Abs further confirmed the expression (Fig. 5B).

Protein expression of the IK1 channel was assessed by the Western blot analysis using polyclonal antibody raised against synthetic peptide corresponding to a region of human IK1 (LYDLQQNLSSSHRALEKQIDTLAGKLDALTELLSTALGPRQLPEPSQQSK, Sigma-Aldrich; cat.# AV35098).

The identity of each isoform preparation was verified by western blot analysis, using polyclonal antibodies recognizing the sequence encoded by exons 1 9 of all HSL isoforms or antibodies specifically recognizing exon A-encoded sequences of HSLbeta or exon T-encoded sequences of HSLtes (Figure 1, B D).

Cox-2 proteins were determined by Western blot analysis using polyclonal antibodies against Cox-2.

Pharmacodynamic assessment of entinostat on histone acetylation status in PBMCs and tumour tissue was performed by western blot analysis using polyclonal antibodies against human histone H3.

followed by staining with SimplyBlue Safestain™ (Invitrogen Ltd).; western blot analysis using polyclonal goat anti-human kappa light chain HRP-conjugated antibody (Ab) (Sigma-Aldrich Company Ltd).

Western blot analysis used polyclonal εPKC, δPKC, p-Akt, Akt, and cytochrome c antibodies, was normalized to ANT (mitochondria) and GAPDH (total and cytosolic homogenates) and was expressed as percent control.

To facilitate the interpretation of the EDC-induced covalent union, the cross-linking kinetics were electro-transferred and revealed by Western blotting analysis using polyclonal anti-gastrokine-1 ("Figure 8", panel B).

Proteins were assessed for purity and quality by Coomassie-stained polyacrylamide gels and by Western blot analysis using a polyclonal rabbit anti-pσ1 (produced in-house) or a polyclonal rabbit anti-OVA Ab (Sigma-Aldrich).

Low levels of SOD expression was detected in B. neotomae strain 5K33 by Western blot analysis using specific polyclonal antibodies.

Subsequent western blot analysis using the polyclonal anti-rTbUNC119 antibody demonstrated predominant expression of TbUNC119 in flagellum fraction, confirming its flagellum localization (Fig. S2).