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Prostacyclin synthease protein levels in endothelial cells were determined by Western blot analysis using an anti-PGI2 synthease rabbit polyclonal antibody.
Expression of HcpA was determined by Western blot analysis using an HcpA-specific mAb, BR-1.
Overexpression was confirmed by Western Blot analysis using an isoform-unspecific antibody (Fig. 3A).
We also examined LDHA protein levels by western blot analysis using an LDHA-specific antibody.
This finding was confirmed by Western blot analysis using an anti-PCNA monoclonal antibody (Fig. 1B).
Bound protein was detected by Western blot analysis using an anti-FLAG antibody.
Western blot analysis using an anti-myc antibody revealed the expression of a 61 kDa protein (Fig. 2B, right).
Fractions containing histone H2B were determined by Western blot analysis using an α-H2B antibody (Active Motif, Cat. No. 39237).
The presence of the fusion protein in sik1flp/flp ES cells was confirmed by western blot analysis using an antibody against the β-galactosidase (Fig. 1C).
Western blot analysis using an anti-V5 tag antibody revealed comparable SYT-SSX1 protein expression among the four infected hMSC populations (fig. 1A).
Western blot analysis using an antibody directed against non phosphorylated p47phox showed that the same amount of proteins was present for all conditions tested.
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