We evaluated by western blot analysis the effects of treatment with Erb-hRNase and Erb-hcAb on the expression of proteins involved in the ErbB2 pathway in JIMT-1 and KPL-4 cells.
Notably, when we evaluated by quantitative real-time PCR and western blotting analysis, the effects of treatment with the different G4 ligands on c-MYC and BCL2 expression in a human melanoma cell line, EMICORON appeared the most effective compound in reducing the mRNA and protein levels of both genes.
On the basis of the western blot analysis, the inhibitory effect of PD98059 on the phosphorylation of ERK1/2 paralleled the downregulation of Cbf α-1 and ALP activities in a similar dose-dependent manner.
Very interestingly, prolonged COX-2 inhibition was associated with a strong impairment of PGE2 production and, as previously demonstrated with western blot analysis, the strongest inhibitory effect was obtained after InvColi-pSTBE infection.
(b) Western blot analysis of the effect of FMNPs on the expressions of cyclin D3, laminin, FAK, fibronectin, and β-actin.
Lysates were prepared for ERK-1 expression by Western blot analysis and the effect of ERK-1 silencing on HCV was assessed by measuring the luciferase gene activity.
These findings suggest that dFdC may still have inhibition impact on RR. Figure 5: Western blot analysis of the effect of dFdC on expression of ribonucleotide reductase subunits M1 (RRM1) and M2 (RRM2), P53-controlled ribonucleotide reductase (P53R2).
To explore the involvement of the Rho and Rac GTPases in lovastatin-mediated effects, changes in distribution of Rho and Rac GTPases were analyzed by Western blot analysis, and the effects of C3-exoenzyme on lovastatin-induced cytoskeletal changes were evaluated by immunocytochemical analysis.
In addition, Western blotting analysis of the effects of the PI3K/Akt pathway inhibition onto FoxO1 expression levels showed that the enhanced FoxO1 downregulation found in KRIT1−/− cells resulted sensitive to the PI3K selective inhibitor LY294002 (Supplementary Fig. S1C,D).
