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Overexpression of LMP1 in LMP1-transfected TW01 cell line was confirmed using RT-PCR and western blot analysis shown in Figures 1A and 1B.
The alkaline Southern blot analysis shown in supplementary Fig S15 was carried out as follows: 3 × 10 GC of viral DNA were extracted from AAV particles.
The results of the Southern blot analysis shown in Fig. 6B demonstrate that the Xmr region is highly methylated in the brain and liver, whereas a considerable proportion of the restriction fragments appear unmethylated in the testis.
As qRT PCR and western blot analysis shown in Figures 6C and D, both ABCG2 and MMP16 expressions were inversely correlated with miR-328 expression in SP and Non-SP cells.
Results of western blot analysis shown in Figure 3C indicate that Rm-HE treatment caused a strong activation of caspase 8 (detected at 4 h) together with the activation of caspases 7, 3 and 9 in a time-dependent manner.
When the mitotic index (derived from those at the highest dose of mAMSA) was plotted against topoisomerase II α expression level derived from the western blot analysis shown in Figure 3, a good correlation was obtained r=0.99.
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Western blot analysis showed in Sur F/F ; Gdf9-Cre mouse oocytes and Sur F/F ; Cyp19-Cre mouse GCs, the Survivin protein was depleted.
This induction of MMP-2 activity was correlated with Northern blot analysis, showing in HUVECs that IGF-II increased the expression of MMP-2 mRNA, while it did not affect that of TIMP-2, a tissue inhibitor of MMP-2.
Real time PCR and Western Blot analysis showed, in both in vitro and in vivo samples, an increase in mRNA and protein levels of anti-apoptotic molecules, such as BCL-w, BCL-xl, and survivin, and a reduction of the pro-apoptotic molecules BAD, BAX and PUMA.
These results agree with those from the western blotting analysis shown in Fig. 1B, and suggest that CnAß-FK may be predominantly expressed in mature follicular keratinocytes and SG cells.
As shown in Figure 4A, CREB1 phosphorylation level was increased by palmitate over the simulation time, which matched the results obtained by western blotting analysis shown in Figure 2.
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