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Growth assays and western blot analysis show that Lpat471 is less active than wild type Lpa; however, its activity is sufficient to cause a growth defect and measurable decrease in lipoylation, making Lpat471 a potentially useful probe in other organisms.
Western blot analysis show that PC-2 is immunoprecipitated along with PC-1 in a specific manner, since an IP using anti-HA antibodies in lysates from wild-type MEFs did not show any PC-2 immunoprecipitation, despite the fact that equal amounts of PC-2 are expressed by both cell lines (Figure 2B).
The immunocytochemistry and Western blot analysis show that C2C12 cells also contain ATOH8 protein.
Our data from Western blot analysis show that USF-1 protein expression is increased in a confluence-dependent manner, while HNF-1α expression is unchanged for both cell lines, suggesting that only USF-1 is correlated with the confluence-dependent increase for CD26 expression.
Finally, our data from immunostaining and Western blot analysis show that the muscle myosin depletion phenotype has an onset in adults, but it does not rule out that the casual defect occurs prior to adulthood, since the genetic deletions are always present.
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Western blot analysis showed that TNFα and GW4869 did not change endogenous LC3-II levels.
Western blot analysis shows that resveratrol increases MKP-1 protein expression in vitro (Fig. 4d).
Immunoprecipitation and western blot analysis shows that ERK inhibition increases NTHi-induced MyD88s protein expression (Fig. 3a).
(b, c) Western blot analysis showed that the transcription factors and stem cell markers were not expressed under normoxia.
Western blot analysis showed that Nrf1 co-fractionates with LSD1 in both 3T3-L1 LSD1 3T3-L1 LSD1(Ctrl) cells (Fig. 3b).
Western blot analysis showed that expression of sEH was greater in male vs. female mice.
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