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Western blot analysis, performed on some significantly changed proteins, validated the 2D results.

Northern blot analysis performed on total RNA from brains of PrPmyc mice confirmed transcription of transgenic PrPmyc (Fig. 1C).

Southern blot analysis performed on the two trkH mutants and on the 4 auxotroph mutants, confirmed that they (Table 2) contained a single chromosomal transposon insertion (Figure S1).

Western blot analysis performed on protein extracts from miR-221 expressing tumors showed a clear reduction of p27 levels, as compared to control samples (Fig. 1F).

Moreover, western blot analysis performed on either isolated intestinal epithelial cells or total colon samples confirmed an increase in caspase-3 processing in TLE-fed, DSS-exposed mice compared to those isolated from DSS control mice (Fig. 7B).

To confirm the changes in EGFR expression, Western blot analysis performed on cell lysates from M031dip, +7, and +19 lines demonstrated that M031+7 and M031+19 lines over-express EGF receptor family proteins, including human epidermal growth factor receptor 2 (Her-2/neu/ErbB2), resulting in increased activation of phosphorylated EGFR (Tyr992) (Fig. 3A).

To detect His6-PfI2 proteins injectedtextractscts, electrophoresis followed by western-blot analysis performed on oocytes extracts.

Western blotting analysis performed on the transfected cells revealed that p-Smad2 levels were lowest in the MAWBP&D group and highest in the Vector group (Fig.  5b).

Western blotting analysis performed on lumbar spinal cord homogenates at the end stage demonstrated a reduction of gp91phox/NOX2 of about 65% in BBG100 and 60% in BBG40 mice, compared with those treated with vehicle (Fig. 4A).

Southern blot analysis was performed on one ΔhacA mutant (NC6.2) and two ΔireA primary transformants (NC7.1 and NC7.2) and confirmed deletion of both genes (Fig.  3).

FACS analysis of GFP expression (always >95%, data not shown) and western blot analysis was performed on polyclonal cells to confirm successful knockdown.