Sentence examples for blot analysis did not from inspiring English sources

Western blot analysis did not detect any SOLD1 protein in BT-1 cell conditioned medium (Fig. 7).

In contrast, although PCR allowed us to amplify TSPO homologous sequences in P. fluorescens MFY70, MFY162, MFN1032, and in P. putida MFN3597, western blot analysis did not detect the expression of bacterial TSPO-like immunoreactive proteins in these micro-organisms.

It was perhaps surprising that L2 and Ex2 exhibited cryptic promoter activity because a Northern blot analysis did not reveal additional RNA species (data not shown), although this incongruity has been observed previously [32].

The differences in plasma membrane vs cytoplasmic levels of Dvl2 observed by western blot analysis did not exactly correlate with the immunohistochemical data (Fig. 2) as we did not see an increase in endogenous Dvl2 in the membrane fraction by western, while we observed increased membrane localization with exogenous Dvl2HA by immunohistochemistry.

Examination of the pooled sera from early and late PD patients and controls by Western blot analysis did not show substantial immune reactivity (data not shown), confirming the ELISA results on the lack of immune responses towards oligomers in the vast majority of healthy and PD individuals.

Southern blot analysis did not reveal any other genes in Atlantic salmon other than zlg.

As 1D Western blot analysis didn't work for our purpose (Fig. 3), we then took a comparative 2D Western blot approach so that comparison of sulfenation signal intensities could be made between corresponding gel spots.

Unchanged EAAT2 protein expression as revealed by western blot analysis does not rule out a posttranslational effect of ceftriaxone on the number of functional transporters and the transport capacity of a single transporter protein in the plasma membrane.

Although western-blot analysis did not reveal the presence of TNW in a kidney control tissue extract, we could observe a slight TNW staining by immunohistochemistry in all 10 control kidney tissues analyzed.

It has been suggested that iron deficiency may result in an increased rate of proteolytic cleavage of the intact and biologically active hormone to inactive C- and N- terminal fragments [ 40, 41], although a recent western blotting analysis did not confirm this hypothesis [ 42].

Mpt5 protein abundance, as determined by western-blot analysis, does not differ in yeast-form cells and constitutively filamentous STE11-4 cellS.(S. Prinz, unpublished).