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Western blot analysis by using an anti-hUbc13 monoclonal antibody further confirms that UBC13 is a candidate housekeeping gene.
To determine whether the cloned Rf4 reduced rpl5-orf352 transcripts, we performed northern blot analysis by using RNA extracted from the leaf blades (Additional file 1: "Methods").
Following insulin stimulation (10−6 M for 5 minutes) and cell lysis, 2 mg of proteins were immunoprecipitated with anti IR alpha-subunit antibody and analyzed by Western blot analysis by using ENPP1 specific polyclonal antibody (N20 Santa Cruz Biotechnology, CA).
Microneutralization antibody-positive serum was subjected to Western blot analysis by using recombinant protein from A/HK/156/97 virus.
To determine the presence of Mu13, nine inbred lines of maize were analyzed by Southern blot analysis by using the Mu13 internal sequence as a probe.
These data were confirmed by immunohistochemistry and western blot analysis by using a collection of 16 monoclonal antibodies for the detection of various chains of laminin.
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To validate the 2-DE results, the differentially expressed proteins were further examined by Western blotting analysis by using commercially available antibodies.
The cell lysates were subject to SDS PAGE and Western blotting analysis by using an anti-Tie2 antibody (sc-324, Santa Cruz Biochemicals).
To confirm the ELISA results, IgG-positive samples were further subjected to Western blot (WB) analysis by using TOSV (isolate H/IMTSSA [ 2 ])–infected cell lysate (4 ).
The deletion of endogenous AaargB was confirmed by Southern blot analysis using DNA probes that were amplified with the primers AaargB3f3 and AaargB3r using pAaargB as a template.
The deletion of endogenous Aaku80 was confirmed by Southern blot analysis using DNA probes that were amplified with the primers ku80R4 and aku80BR1 using pAaku80 as a template.
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