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OXP induced apoptosis was confirmed with Western blot analysis by measuring PARP (Poly-ADP-ribose polymerase) cleavage and DNA damage by H2AX (Histone 2AX) phosphorylation [ 11, 53, 54].
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Production of the individual proteins from the polycistronic construct was confirmed by Western blot analysis and by measuring the production of β-carotene.
As reported, the concentrations of the secreted enzymes in the different cultures were calculated by comparing the extracellular fraction to serial dilutions of purified enzymes, both by dot-blot analysis and by measuring reducing sugar formation on phosphoric acid-swollen cellulose (PASC) or xylan substrates.
Importantly, Mcl-1 depletion increased apoptosis in JAK2V617F mutant SET-2 cells and sensitized the cells to NVP-BSK805-induced cell death as assessed by Western blot analysis and measuring the sub-G1 cell fraction by flow cytometry.
The difference was apparently not due to higher overall levels of HLA-E protein in these cells, as evidenced by Western blot analysis measuring relative levels of the two allelic heavy chains as roughly equal (Fig. 2).
We further validated the findings of the RTK array kit by western blot analysis to measure the levels of phosphorylated HER-2, and HER-3, as well as that of MAPK and Akt, two major molecules mediating cell signal transduction downstream of EGFR.
The results of Western Blot analysis were quantified by measuring the relative intensity compared to the control by using Kodak Molecular Imaging Software (Version 4.0.5, Eastman Kodak Company, Rochester, NY, USA) and represented in the relative intensities.
A potential limitation of this study is that tissue mRNA expression or protein activation by western blot analysis was not measured.
The serum expression levels of both MYOM3 fragments were determined by Western blot analysis and CK assessed by measuring its enzymatic activity (Fig. 2).
Gene silencing effect was evaluated by western blot analysis and apoptosis was measured by annexin V/PI staining method.
The mean length of the TRFs, determined by Southern blot analysis, was used to measure LTL on DNA extracted from peripheral leukocytes.
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