Sentence examples for blot analysis by digesting from inspiring English sources

Next, we performed Southern blot analysis by digesting genomic DNA with multiple restriction enzymes to further validate the structure we produced from our cloned sequences and from the information derived from the fission yeast genomic database of S. japonicus var.

The mobility of the unglycosylated PrPSc band and the glycoform ratio of proteinase K digested PrPSc were similar to those in BSE when assessed by Western blot analysis by using monoclonal POM-1 antiprion antibody as described (7 ).

The Southern blot analysis was performed by digesting the genomic DNA with restriction enzymes which cut only once in the transfected vector (to discriminate concatamers).

These clones were also checked for the correct integration of the phleo replacement cassette by Southern blot analysis of SalI digested genomic DNA followed by hybridization with the phleo probe.

After double selection, clones were screened by Southern blot analysis of HindIII digested genomic DNA using 1.6 kb fragment from intron 1 of mCgb as probe.

BAC co-integrates were screened for successful recombination by PCR and Southern blot analysis of Hind III digested BAC DNA using radiolabeled 'A' box as a probe.

Homologous recombination of the "long arm" was analyzed by Southern blot analysis of genomic DNA digested with BamHI with P32-labeled probe "L".

To demonstrate that the DNA circles were precisely inserted by Flp into the FRT docking site, we first analyzed five representative clones by southern blot analysis of genomic DNA digested with Xba I, which cuts within the duplicated FRT sequences.

In some experiments, genotypes were confirmed by Southern blot analysis of the EcoRV digested genomic DNA from C2CR mice, probed with a P-labelled DNA fragment comprising the 2.7-kb BamH1 fragment of the transgene.

Right: results of Southern blot analysis using genomic DNA digested with Nhe I and Hin dIII.

N, restriction sites of Nco I. (b) Results of the Southern blot analysis using genomic DNA digested with Nco I (N).