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Flagellin amounts in the expectorations were quantified by Western Blot analysis by comparing the band intensity to a standard curve of purified Pseudomonas flagellin, purified as described previously [ 26].
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Moreover, it showed the availability of semi-quantitative analysis by comparing Western blot.
To determine whether a correlation existed between ELISA values and reactivity by Western blot analysis, we compared data obtained by ELISA with Western blot results by logistic regression.
PrPRES concentration was estimated by western blot analysis, comparing in the same blot the signal intensity of different dilutions of the purified protein with known concentrations of the recombinant mouse PrPC, purchased from Prionics Inc (Zurich, Switzerland).
Dominant var gene expression detected by qRT-PCR was verified as both full-length and dominant by northern blot analysis, comparing specific probes for each dominant gene vs a generic exon 2 probe (data not shown).
These results were further confirmed by a Western blot analysis comparing discs exclusively composed of PTEN mutant tissue with discs bearing wild-type clones under normal food conditions.
In seven fibroblast cultures, FGFR2 protein levels were quantified by Western blot analysis and compared with the FGFR2 mRNA levels.
The exosome-based delivery of siRNA to cells was determined by Western Blot analysis and compared with direct transfection of the same siRNA to cells.
By western blot analysis, we compared levels of proteins phosphorylated on tyrosine as well as cadherins and integrins in human melanoma cells overexpressing or not Nck2.
Reduced expression of nm23-H1 protein in the antisense-transfected (AS) clones was found by Western blot analysis as compared to wild-type YES-2 and YES-2/Neo (clone transfected with the neomycin resistance gene alone).
Remarkably, the expression of Abi1 in p185wt cells transduced with Abi1 shRNA was reduced to a level undetectable by western blot analysis, as compared with p185wt cells transduced with scrambled shRNA.
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