Sentence examples for blot analysis based on from inspiring English sources

40 μg of total protein was used for Western blot analysis based on standard procedures.

To distinguish between hemizygous and homozygous rhodopsin knockout mice, we performed Southern blot analysis based on a previous protocol [67].

As described previously, three hydrogel compositions were chosen for Western blot analysis based on their dominant method of cross-linking as well as their astrocyte adhesion response: A5/C0/G0/Ca22, A5/C125/G1/Ca24, and A5/C25/G01/Ca20.

After confirming selected PCR results by Southern blot, a MLST analysis based on those PCR/Southern blot results was then applied to characterize the genetic backgrounds of chromosomal cpe isolates vs. plasmid cpe isolates.

To define the conditions when c-FLIPL has a pro-apoptotic action, an ODE-based model of the DISC was developed by Fricker et al. The model was validated using quantitative western blot and single-cell analysis based on experiments in HeLa cells overexpressing CD95 (HeLa-CD95 cells).

The linear regression line between mean telomere length (measured by terminal restriction fragment analysis based on Southern blotting) and the T/S ratio (measured by the quantitative PCR based method) was obtained, as described previously (14, 16), and used for the calculation of the corresponding telomere length in base pairs (bp) from the T/S ratio measured in each subject.

From 1995 through 1997, one M. tuberculosis isolate from each patient with MDRTB in New York City was sent to the Public Health Research Institute Tuberculosis Center, where DNA fingerprint analysis, based on IS6110 Southern blot hybridization pattern, was performed by using a standardized protocol (16).

DNA analysis based on IS 6110 Southern blot hybridization was performed at the Public Health Research Institute with previously described methods (28, 29 ).

Further analysis based on RT-PCR and Western blot indicated it as CD44 variant 4 (CD44v4) (Fig. 5).

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The methods for protein extraction, followed by western blot analysis, were based on protocols from previous studies22,48.