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To confirm differential gene expression, a combination of relative real time RT-PCR and northern blot analyses were utilized with all 7 animals from Exp. 1 (fed Zn150 or Zn2000without phytase), and all 23 animals of Exp.2, which were fed the six dietary treatments.
Similar(59)
Immunoflourescence analyses were utilized, as commercially available anti-Clc-1 antibodies are unable to detect chloride channels by Western blot analyses.
Both visual and statistical analyses were utilized.
Descriptive statistical analyses were utilized and qualitative analysis conducted.
Western blot analyses were performed utilizing standard procedures.
One-dimensional Western blot analyses were performed utilizing standard procedures.
Right: Southern blot analyses were performed as in (b).
Western blot analyses were performed as previously described [25].
RNA gel blot analyses were performed with 5 μg of total RNA.
Northern blot analyses were performed essentially as described previously (Sambrook and Russell 2001).
Western blot analyses were performed as previously (Park et al. 2008).
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