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Southern and Western blot analyses were used to confirm proper targeting and complete loss of DCC protein, respectively [26].
Altogether 16 patient sera that had shown reactivity in the 1-dimensional Western blot analyses were used for the identification of antigens in the 2-dimensional Western blots.
Quantitative Northern blot analyses were used to determine the level of mature miRNA expression and processing of the pre-miRNA and mature miRNA.
Quantitative Northern blot analyses were used to define the levels of mature miR-181a and miR-181c, as well as the sizes of the mature miRNAs and the levels of the pre-miRNAs produced from various mir-181a-1/c mir-181a-1/c mir-181a-1/c 23 cells (Fig. 6A, 6B, 6E, and S8).
RT-PCR and western blot analyses were used to estimate the degree of integrin KD.
Western blot analyses were used to quantify RyR expression as previously described (Roegge et al. 2006).
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To examine the role of the mitochondrial pathway in BSO plus estradiol-induced apoptosis, western blot analyses was used to measure Bax, Bcl-2, phosphorylated Bcl-2, and Bcl-xL protein levels in MCF-7 2A MCF-7 2Allowing treatment with 1 nM estradiol alone, 100 μM BSO, or BSO plus estradiol for 48 h.
Immunofluorescence, Western blot and apoptosis analyses were used to investigate their regulation of oxidative stress and mitochondrial function.
Northern blot and ELISA analyses were used to determine IL-8 RNA expression and cytokine secretion.
Both Western blot and immunohistochemical staining analyses were used to test the expression of Hpa in HepG2, SGC-7901, MKN45, MCF-7, SW480, and U2OS cells.
In vitro direct and competitive pull-down studies as well as western blot and RT-PCR analyses were used to validate predictions.
More suggestions(17)
blot analysis were used
blot screens were used
blot analyses were summarized
blot methods were used
blot profiles were used
blot analyses were stored
blot hybridisations were used
blot analyses were selected
blot analyses were conducted
blot analyses was used
blot analyses were performed
blot data were used
blot analyses were carried
blot analyses were shown
blot experiments were used
blot procedures were used
blot techniques were used
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