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Samples for Western blot analyses were stored at −80°C until ready for analysis.
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Tissue for biochemical assays and western blot analyses were isolated separately and were stored at −80°C until subsequent analyses.
Right: Southern blot analyses were performed as in (b).
Western blot analyses were performed as previously described [25].
RNA gel blot analyses were performed with 5 μg of total RNA.
Northern blot analyses were performed essentially as described previously (Sambrook and Russell 2001).
Western blot analyses were performed as previously (Park et al. 2008).
Protein blot analyses were performed.
Representative blots of three or more independent quantitative Northern blot analyses were shown.
Western blot analyses were performed exactly as described previously [30].
Western blot analyses were performed in standard conditions.
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