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Representative blots of three or more independent quantitative Northern blot analyses were shown.
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Results from Western blot analyses are shown in panels B and D. Clearly, F1-40 bound to the light-chain fragment (Lc) and to subfragment L1, but binding to L2 was not detected (Figure 2, Panel B).
Representative full-length blots from Western blot analyses are shown in Fig. S2.
Western blot analyses are shown (Fig. 4a- c), along with quantification of Fc content (Fig. 4d) and secretion rate (Fig. 4e).
Cell cycle and the western blot analyses were repeated three times, and the results of representative experiments are shown.
Altogether 16 patient sera that had shown reactivity in the 1-dimensional Western blot analyses were used for the identification of antigens in the 2-dimensional Western blots.
All Western blot analyses were performed in triplicates for all available mutants (PINK1: p.Q456X and p.V170G; Parkin: p.V324fsX434 and p.R245fsX253) and two controls, and representative blots are shown in the figures.
Right: Southern blot analyses were performed as in (b).
Western blot analyses were performed as previously described [25].
RNA gel blot analyses were performed with 5 μg of total RNA.
Northern blot analyses were performed essentially as described previously (Sambrook and Russell 2001).
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