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Total protein extracts for Western blot analyses were prepared by homogenization of frozen tissues in SDS/PAGE sample buffer followed by brief sonication and boiling.
Western blot analyses were prepared as previously reported.
Protein extracts for Western blot analyses were prepared as follows: five dehulled seeds were ground with 1 mL of extraction buffer (500 mM NaCl in 50 mM Tris-HCl buffer, pH 7.0) using mortar and pestle.
Cell and tissue lysates for western blot analyses were prepared as described in Reference (11).
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Samples used for Northern blot and microarray analyses were prepared by culturing A. salmonicida wild-type and a spf deletion mutant strains at 12°C in ASMM without any added carbon source to OD600 nm 0.4.
Whole cell protein lysates were prepared and western blot analyses were conducted as described previously [ 22].
Total protein extracts from striatum and ventral midbrain were prepared and western blot analyses were performed as described previously.
Western Blot analyses were performed on lysates prepared from cancer cell lines and ovarian cancer spheres to confirm the immunohistochemistry findings.
Western blot analyses were performed on cell lysates prepared from MDA-MB-231 and Hs578T cell lines as described previously [ 12].
Western blot analyses were performed by separating nuclear extracts prepared as described in [ 39] from undifferentiated pre-confluent and differentiated 10-days post-confluent Caco-2 cells on a NuPAGE 12% Bis-Tris gel (Invitrogen).
Western blot analyses were performed on right cortex of the brain prepared from incision, sCHI and rCHI rats.
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