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Although the Westerns blot analyses were not quantitative, we noticed that a higher level of expression of individual peptides was achieved by co-infection as compared to infection with individual viruses.
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Due to asynchronous macronuclear differentiation in mass cultures, performance of quantitative normalized Western blot analyses was not possible.
Since commercially available ELISA were unable to detect these proteins within the given detection limit (data not shown), Western blot analyses were used.
Western blot analyses were performed utilizing standard procedures.
Western blot analyses were performed according to standard procedures.
Western blot analyses were performed as previously described33.
Histone extraction and Western blot analyses were performed from 10 larvae as described previously62.
Right: Southern blot analyses were performed as in (b).
Western blot analyses were performed as previously described [25].
Western blot analyses were performed with polyclonal antibodies against two isoforms of rat arginase (I and II) and iNOS.
RNA gel blot analyses were performed with 5 μg of total RNA.
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