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Western blot analyses were conducted on CL, cortex and granulosa/theca samples and IHC was performed on CL tissues using D1r or D2r specific antibodies.
Western blot analyses were conducted.
Western blot analyses were conducted as described previously [ 22].
Western blot analyses were conducted as previously described [ 19, 20].
Western blot analyses were conducted as previously described [ 30].
Western blot analyses were conducted after separation by SDS-PAGE electrophoresis and transfer to nitrocellulose membranes.
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Initially both Southern blot and PCR analyses were conducted to confirm genotype.
Western blot and immunocytochemical analyses were conducted to determine induction of target proteins.
Cell fractionation, Western blotting and DiGE analyses were conducted as previously described [18].
Western blot and RT-PCR analyses were conducted to compare protein and mRNA expression, respectively, of ERα, HER2, and HIF-1α (inducible HIF-1 subunit) in AI-resistant versus AI-sensitive cells.
Quantitative densitometric measurement of western blotting was performed using Image J. Statistical analyses were conducted by Student's t-test or Welch's t-test.
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