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Genomic DNA and total RNA isolation, and Northern blot analyses were carried out as described [35].
Western blot analyses were carried out as previously reported [18], [21].
Protein extraction and western blot analyses were carried out as described previously [46].
To confirm this speculation, western blot analyses were carried out using anti-modified histone H3 antibodies, an anti-histone H3 antibody and an anti-spTFIIEα antibody as an internal control.
Western blot analyses were carried out on PVDF membranes using the following primary antibodies and dilutions: mouse anti-ILK (1∶200), rabbit anti-Src (1∶200) and rabbit anti-SPARC (1∶500) - all from Santa Cruz Biotechnology, Inc. (Delaware, CA, USA) - and mouse anti-β-actin (1∶1000) from Millipore (Temecula, CA, USA).
Western blot analyses were carried out using 8% SDS polyacrylamide gels which were electroblotted onto PVDF membranes (Biorad, Italy); filters were blocked for 1 h in TBS-T buffer (100 mM Tris-HCL; 0.9% NaCl, 0.1% Tween 20, pH 7.4) containing 5% non-fat dry milk.
Similar(33)
Western blot analyses was carried out by probing with acetylated histone [ H3 Lysine 14, H4 Lysine 12,H4 Lysine 16] and histone H3 and H4 antibodies.
Immunoprecipitation and Western blotting analyses were carried out as previously published (Yuasa-Kawada et al., 2009a).
Far-Western and Western blotting analyses were carried out as previously described [ 6, 7, 23].
Cellular proteins were extracted and separated on SDS-PAGE gels, and Western blotting analyses were carried out as described previously(19).
Mass spectrometry and candidate western blotting analyses were carried out on FCD-2 immunoprecipitates from untreated and cisplatin-treated whole worm extracts.
More suggestions(16)
blot analyses was carried
blot analyses were conducted
spot analyses were carried
blot assays were carried
blot analysis were carried
blot transfers were carried
blot experiments were carried
blot analyses were done
blot analyses were stored
blot analyses were prepared
blot hybridizations were carried
blot analyses was carried
blot analyses were performed
blot analyses were shown
blot analyses were repeated
blot analyses were used
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