Sentence examples for blot analyses using the from inspiring English sources
We carried out northern blot analyses using the sequences of OsPDS and OsSLR1 Gene Specific Tags (GST) as probes to hybridize sequentially the same membranes.
The purity of the protein was determined by SDS-PAGE with Coomassie Blue staining and Western blot analyses using the anti-truncated SDH domain of LKR/SDH mouse sera.
To elucidate the expressions of F3H, F3′H, and F3′5′H, we next performed northern blot analyses using the same RNAs.
We then performed Western blot analyses using the same developmental stage samples used for SDS-PAGE and blotting as described above.
Western blot analyses using the anti-IFT27 antibody showed a single band migrating at a position close to the predicted size of 20 kDa in wild-type cells.
To obtain proof that H3.7 is targeted by the specific acetylation that was detected using the H3K36ac antibody (GVKacKPHR), we performed Western blot analyses using the same samples as described above, in combination with PTM-specific antibodies.
Total RNA was isolated from the two youngest expanded leaves or shoot apices and subjected to real-time RT-PCR or Northern (RNA) blotting analyses using the constitutively expressed EF1α and/or Actin2 genes as references.
Southern blot analyses, using as probes the 560-bp LTR or the gag-pol polyprotein sequence of 605 deduced amino acids, uncovered several bands from all restriction enzymes, suggesting that TORE1 or related elements are ubiquitously present in the torenia genome.
In a similar way, the use of biotinylated NAD+ or etheno-NAD+ (an NAD+ analogue with an additional penta-ring, i.e. etheno-group, on the adenosine moiety) allows detection of the target protein by western blot analyses using streptavidin or the etheno-adenosine-specific monoclonal antibody 1G4, respectively (Krebs et al. 2003).
Northern blot analyses using probes generated from the full-length Atp7a cDNA revealed several specific hybridization bands, all of which were more intense in RNA samples extracted from duodenal enterocytes isolated from iron-deficient rats.
