Sentence examples for blot analyses using an from inspiring English sources
In order to determine if ADAM-10 plays a role in the increased expression of the soluble form of betacellulin in the retinas of diabetic mice we performed western blot analyses using an ADAM-10 antibody.
Following 12 h induction on galactose medium, Western blot analyses using an eIF2α 51-phospho-specific antibody confirmed that these kinases phosphorylated eIF2α on Ser51.
We stimulated monolayer HUVECs with either VEGF165 or bFGF for various time periods between 0 and 18 h, and subjected lysates to Western blot analyses using an affinity purified anti-LPP3-cyto antibody (Fig. 1A).
To confirm their effects on PKA activity, we performed Western blot analyses using an antibody that detects proteins containing a phospho-serine/threonine residue with arginine at the -3 and -2 positions, which are typically found on proteins phosphorylated by PKA.
Western blot analyses using an anti-Flag-tag antibody showed a widespread distribution of the AAV9-SynI vector in the central nervous system, including the spinal cord, cortex, cerebellum and olfactory bulb of AR2 mice intravenously injected with AAV9-Flag-hADAR2 (AAV).
Following immunoprecipitation with a α-Myc antibody, Western blot analyses using an α-Flag antibody showed that a C-terminally truncated Smad7 1-408) accompanied a detectable amount of Flag-HDAC-1, whereas further truncated fragments (1 380 and 1 259) did not (Fig. 1C).
Furthermore, GLUT4 homologues could not be detected in chicken tissues by genomic Southern blot analyses using a rat GLUT4 cDNA probe.
The molecular sizes of the proteins were confirmed by western blot analyses using a PEDF polyclonal antibody generated in our lab as shown in Figure 7B (Lanes1, 2: uncleaved and cleaved proteins respectively).
Successful deletion of the PaMth1 gene was verified in hygromycin B resistant transformants via Southern blot analyses using a PaMth1- and hygromycin B (hph) specific probe, respectively.
To examine the impact of both miRs on the protein expression of MAGE-A1, we performed transfection studies using mimics and inhibitors of let-7b and miR-202 and western blot analyses using a MAGE-A1-specific antibody.
Moreover, the stable integration of the gluc gene into the genome of Eudorina as well as the copy number of the integrations was investigated by Southern blot analyses using a gluc-specific probe.
