Sentence examples for blot analyses using a from inspiring English sources

Furthermore, GLUT4 homologues could not be detected in chicken tissues by genomic Southern blot analyses using a rat GLUT4 cDNA probe.

The molecular sizes of the proteins were confirmed by western blot analyses using a PEDF polyclonal antibody generated in our lab as shown in Figure 7B (Lanes1, 2: uncleaved and cleaved proteins respectively).

Successful deletion of the PaMth1 gene was verified in hygromycin B resistant transformants via Southern blot analyses using a PaMth1- and hygromycin B (hph) specific probe, respectively.

Moreover, the stable integration of the gluc gene into the genome of Eudorina as well as the copy number of the integrations was investigated by Southern blot analyses using a gluc-specific probe.

Also, Southern blot analyses using a kinase domain-specific probe of each gene revealed that NbMAPKKKβ and NbMAPKKKγ exist as single-copy and multiple-copy genes, respectively, in the N. benthamiana genome (Additional file 1: Figure S1).

Upon the addition of TET to the medium, the extent of RNAi-targeting Tbβ-MPP was followed by northern and Western blot analyses using a labeled probe and rat polyclonal anti-Tbβ-MPP antibody, respectively.

In order to determine if ADAM-10 plays a role in the increased expression of the soluble form of betacellulin in the retinas of diabetic mice we performed western blot analyses using an ADAM-10 antibody.

Following 12 h induction on galactose medium, Western blot analyses using an eIF2α 51-phospho-specific antibody confirmed that these kinases phosphorylated eIF2α on Ser51.

We stimulated monolayer HUVECs with either VEGF165 or bFGF for various time periods between 0 and 18 h, and subjected lysates to Western blot analyses using an affinity purified anti-LPP3-cyto antibody (Fig. 1A).

To further validate that the slower-migrating AO7T bands represent ubiquitylated forms of the proxy substrate, we repeated the UbK0 time course using nonradiolabeled HA-AO7T and performed Western blot analyses using an anti-Ub antibody and an anti-HA antibody.

Following immunoprecipitation with a α-Myc antibody, Western blot analyses using an α-Flag antibody showed that a C-terminally truncated Smad7 1-408) accompanied a detectable amount of Flag-HDAC-1, whereas further truncated fragments (1 380 and 1 259) did not (Fig. 1C).