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Results from Western blot analyses are shown in panels B and D. Clearly, F1-40 bound to the light-chain fragment (Lc) and to subfragment L1, but binding to L2 was not detected (Figure 2, Panel B).
Representative full-length blots from Western blot analyses are shown in Fig. S2.
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Representative blots of three or more independent quantitative Northern blot analyses were shown.
The results of SDS-PAGE electrophoresis and Western Blot analyses are shown in Figure 2. The presence of enzymes from NTPDase and NPPase families in porcine CSF was confirmed by their molecular mass as well as Western Blotting analyses.
By zymographic and Western blot analyses, STE was shown to inhibit the activities and expression of MMP-9.
Using Western blot analyses, FCY was shown to influence the expression levels of pro- and anti-apoptotic proteins; these results were confirmed by caspase activity assays.
Cell cycle and the western blot analyses were repeated three times, and the results of representative experiments are shown.
Altogether 16 patient sera that had shown reactivity in the 1-dimensional Western blot analyses were used for the identification of antigens in the 2-dimensional Western blots.
All Western blot analyses were performed in triplicates for all available mutants (PINK1: p.Q456X and p.V170G; Parkin: p.V324fsX434 and p.R245fsX253) and two controls, and representative blots are shown in the figures.
For each cell line, measurements from at least four replicate blots were analysed (two representative blots are shown in Fig. 3).
Right: Southern blot analyses were performed as in (b).
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