Sentence examples for blot against from inspiring English sources

Goat anti-mouse HRP conjugate (A4416, Sigma-Aldrich) was used as secondary antibody to probe the blot against anti-CA125 MAb and mouse anti-β actin IgG at 1 5,000 dilution for 1 h at room temperature.

Specifically, we used an insulin ELISA and Western blotting against insulin, proinsulin, Syntaxin-6, and p18 to determine the iISG and mISG fractions, and we used Western blot against phogrin, β-granin, Bip, TGN46, GAPDH, CPE, CoxIV, and p62 to determine the purity of our enriched fractions.

They did not come, as Carberry and Adams took the game away, collecting 19 fours - plus two sixes for Carberry - between them, with the only blot against the Hampshire pair was their failure to make three figures, thanks to a double-strike from spinner Adam Riley.

For western blot against RIZ1 but not RIZ2, we used the abcam RIZ1 antibody ab9710.

Antibody specificity was verified by western blot against OP-9 cell lines expressing Notch ligands (provided as a generous gift from Dr. John Lowe)[15].

As shown in Figure 1A, only the phospho-peptide antigen specifically blocks reactivity for each antibody by western blot against Hela cell extracts.

The generated mutants were checked by sequencing and also by blotting against anti-GST antibodies (GE Healthcare Life Sciences).

The wild type E2s and its mutants were subjected to non-reducing SDS-PAGE and Western blotting against 8H3.

This method generated highly enriched fractions of iISGs and mISGs as demonstrated by Western blotting against different intracellular organelle markers.

A subset of these samples was studied by Western blotting against HeLa cell extract.

This was consistent with the Western blots against Hu (Figure 3).