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Parasites per ml of tail blood were enumerated using a haemocytometer.
Leucocytes from the lung and blood were enumerated using flow cytometry.
Circulating tumour cells in 7.5 ml of blood were enumerated using the CellSearch system (Janssen Diagnostics, LLC, Raritan, NJ, USA).
Peripheral blood mononuclear cell (PBMC) populations from whole blood were enumerated using flow cytometric analysis during the first 2 cycles of treatment.
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Peripheral blood mononuclear cells (PBMCs) were isolated, and ASCs were enumerated using an ELISPOT assay.
Leukocytes, neutrophils and platelets were enumerated using a Malassez cell after hypotonic red blood cell lysis.
Red blood cells were lysed in Tris-buffered ammonium chloride (pH 7.2) buffer, and leukocytes were enumerated using a Coulter counter.
Cultivable fungi were enumerated using plate counts.
After 7 hrs% of PI- positive cells were enumerated using celigo cytometer.
Cells were enumerated using a Leica fluorescent microscope (DM4000B, Germany).
Colonies were enumerated using OpenCFU software (Geissmann 2013).
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