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DNA was extracted from whole blood using standard methods after written informed consent.
Genomic DNA was extracted from whole blood using standard methods.
Patient DNA was obtained from peripheral blood using standard methods.
Genomic DNA was isolated from whole blood using standard methods [22].
DNA from patients and controls was obtained from peripheral blood, using standard methods.
10 Genomic DNA was isolated from peripheral blood using standard methods.
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Genomic DNA was isolated from white blood cells of total blood using standard method (phenol/chloroform).
Genomic DNA was extracted from buffy coats prepared from EDTA blood samples using standard methods, principally with the Qiagen blood mini kit (Qiagen, Crawley, UK).
23 Blood samples were collected at initial evaluation and DNA was isolated from peripheral blood lymphocytes using standard methods.
DNA was extracted from peripheral blood samples using standard methods.
DNA used for PCR amplification was extracted from venous blood leukocytes using standard methods.
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