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Engraftment and disease progression was monitored by flow cytometric enumeration of the proportion of human versus mouse CD45+ (%hucells) cells in peripheral blood using established procedures [ 2, 10].
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A platinum (Pt) protective layer was deposited over a region of interest (ROI) using established procedures.
The network reconstruction was converted to a constraint-based model using established procedures [ 13].
The probe was hybridised to S. mansoni chromosomes using established procedures [ 78].
We have used established procedures to test urinary concentrating and diluting capacity [ 21].
Use established procedures and training to get the person back from the brink.
We handled, centrifuged, and stored blood samples using established protocols [28].
DNA was isolated from 5 ml of blood plasma and bisulfite converted [ 26] using previously established procedures (for detailed information see Additional file 1).
Proteolytic activity of PepDΔTM against potential substrates was determined using previously established procedures [12].
The glass nanopore membrane (GNM) was fabricated using previously established procedures.
Hippocampal morphometry, the analysis of hippocampal shape, was performed using an established procedure [23].
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