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DNA was extracted from whole blood using a specific kit (DNeasy; Qiagen UK Ltd) and the DNA was tested for the presence of the GYS1 R309H mutation using the restriction fragment polymorphism assay as reported previously [8].
Genomic DNA was extracted from whole blood using a specific DNA preparation kit (QIAamp Blood Mini Kit; Qiagen, Valencia, CA, USA), according to the manufacturer's instructions.
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Secreted Cav-1 can be reproducibly detected in peripheral blood using a sensitive and specific immunoassay.
Secreted Cav-1 can be reliably and reproducibly measured from peripheral blood using a Cav-1-specific ELISA.
Secreted Cav-1 can be measured in peripheral blood using an ELISA that is sensitive, specific, and reproducible.
Blood transfusions were identified using a specific code introduced in the database in 1994 (blood transfusion rates were thus unavailable for 1991 to 1993).
The growth of pancreatic tissues was determined in the SCID recipient mice by monitoring blood levels of pig insulin using a specific ELISA, previously shown to correlate with implant size[8].
The concentration of HI antibodies in the blood (HI titre) is measured using a specific immunological assay [ 2].
DNI (the difference in leukocyte subfractions identified by myeloperoxidase and nuclear lobularity channels) was determined using a specific blood cell analyzer.
The heparinized blood samples were tested by using a specific qPCR; samples from only 1 patient were positive for C. burnetii.
Real-time PCR of peripheral blood, using reagents specific for a patient's particular antigen receptor gene rearrangement, has also been used for remission status monitoring and relapse detection in canine LSA [ 43, 44]; however, this is labor and time intensive and requires the generation of custom tools for each patient, and would also not be suitable as a screening test.
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